双峰驼隐睾组织结构分析

doi:10.11843/j.issn.0366-6964.2016.05.017

Abstract

Abstract:

The histological and ultrastructural features of testis tissue between the healthy Bactrian camels and the bilateral cryptorchid Bactrian camels were compared at the same ages.The Bactrian camel scrotal testis and cryptorchidism testis of 2 years old were prepared for light and electron microscopy by histochemistry and transmission electron methods and IPP (Image-Pro Plus) statistics methods were used to identify the characteristics index of seminiferous tubule in the both testis.The observations made with the light microscope showed that the bilateral cryptorchid Bactrian camels with 1-3 layers seminiferous epithelium in seminiferous tubules of much smaller diameter（P<0.05），thus accounting for the interstitial area and the ratio of interstitial area and bureaucratic area were significantly increased（P<0.01）.Electron micrographs of seminiferous tubule showed that the junctional complex between adjacent Sertoli cells，and also the intercellular canaliculi and associated desmosomes between adjacent primary spermatocyte were obviously observed in the Bactrian camel scrotal testis，but in the testis of the bilateral cryptorchid Bactrian camels，the lamina propria of seminiferous epithelium were hyperplasia which specifically associated with hemidesmosomes.However，there were rudimentary desmosome between adjacent immature Sertoli cells and the primary spermatocytes were rarely found in the tubular lumen with cytoplasmic bridge and desmosome between adjacent Sertoli cells and primary spermatocyte.The Leydig cells of the scrotal testis note the typical features of granular reticulum in the cisternae in direct continuity with this type of agranular reticulum，but in the cryptorchidism Leydig cell，the background cytoplasm were crowded with swelling mitochondria and reticulum were not obvious.The capillary vessel of cryptorchid Bactrian camels testis was smaller and the epithelial basal of the lamina was not clear，and the junction were observed between adjacent microvessel endothelial cells.Taken together，the abnormal proliferation of the Sertoli cells and the arrest of spermatogenesis were the main characters in cryptorchidism seminiferous tubule，and the blood-testis barrier，which affected by the immature Sertoli cells connection with the basement membrane，the defects of the ectoplasmic specialization in Sertoli cells and the rudimentary desmosome adjacent between the Sertoli cells and primary spermatocyte，in addition，the paucity of mixed endoplasmic reticulum and the differences in the development of smooth endoplasmic reticulum apparent in the Leydig cells of the cryptorchid testis could serve as a meaningful index for the further research of the reticulum involved in steroid biosynthesis may be related to the stage of different ages.

CLC Number:

S852.162

YUAN Li-gang，QU Ya-ling，LI Cong，LU Yu-rong，YAN Zhen-long. The Histologic and Ultrastructural Characteristics of the Bactrian Camel Testis in Cryptorchidism[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(5): 993-1000.

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The Histologic and Ultrastructural Characteristics of the Bactrian Camel Testis in Cryptorchidism

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